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PathScan(R) RTK Signaling Antibody Array Kit (Chemiluminescent Readout)
品牌:Cell Signaling Technology
¥7893
咨询公司新闻/正文
1186 人阅读发布时间:2020-04-16 13:02

当对小鼠样本进行 IHC 实验时,CST 更推荐您使用兔单克隆抗体而不是鼠单克隆抗体,为什么,这么做有什么好处?3 步让您了解原因!
使用小鼠单克隆抗体会造成小鼠组织结果无法解读
如经适当验证,小鼠单克隆抗体会特异性地对目的靶标染色,但数据解读很可能因使用抗小鼠 IgG 检测试剂(二抗)同时能检测内源性小鼠 IgG 而受到影响。如图1,依次使用一种 α-平滑肌肌动蛋白(α-SMA)小鼠单克隆抗体和一种抗小鼠 IgG 检测试剂对石蜡包埋的小鼠肺细胞进行免疫组织化学 (IHC) 分析,结果显示出预期的特异性染色,但由于存在抗小鼠 IgG 二抗,还显示出背景染色过度。避免小鼠 IgG 背景的唯一办法是在你的实验步骤中添加费力的步骤,或换用一种非啮齿类抗体,例如联合使用兔单克隆抗体和抗兔 IgG 的二抗。

图1 ,注:小鼠 IgG 二抗导致背景染色过度。
抗小鼠 IgG 二抗会造成小鼠抗小鼠背景染色
使用小鼠一抗需要抗小鼠 IgG 二抗或检测试剂,这同时会对一抗和小鼠组织样品的 IgG 蛋白进行染色。为了让您直观地观察,图2展示的是在没有一抗的情况下,使用 SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125 对石蜡包埋的小鼠肺细胞进行 IHC 分析。

兔单克隆抗体无需额外的实验步骤即可产生可解读的小鼠组织结果
使用在小鼠组织中经 IHC 验证的兔单克隆一抗可克服小鼠抗小鼠染色,并发现明确且可解读的结果。图3 所示的是使用 α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb #19245 对石蜡包埋的小鼠肺细胞进行的 IHC 分析。

| 产品名称 |
货号 |
应用 |
反应性 |
| 细胞生物学 |
|||
| Bax (D3R2M) Rabbit mAb (Rodent Preferred) |
14796 |
WB、IP、IHC-P |
M、R |
| N-Cadherin (D4R1H) XP® Rabbit mAb |
13116 |
WB、IP、IHC-P、IF-IC |
H、M |
| CD44 (E7K2Y) XP® Rabbit mAb |
37259 |
WB、IHC-P |
H、M、R |
| EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred) |
71655 |
WB、IP、IHC-P、 IF-F、IF-IC、F |
M |
| EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) |
93790 |
WB、IP、IHC-P |
H、M、R |
| HER3/ErbB3 (D22C5) XP® Rabbit mAb |
12708 |
WB、IP、IHC-P、 IF-IC、F |
H、M、(R) |
| Integrin ß3 (D7X3P) XP® Rabbit mAb |
13166 |
WB、IHC-P |
H、M |
| Keratin 5 (E2T4B) XP® Rabbit mAb (IHC Preferred) |
71536 |
IHC-P |
H、M |
| Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated) |
12202 |
IHC-P |
M |
| Olfm4 (D6Y5A) XP® Rabbit mAb (Mouse Specific) |
39141 |
WB、IP、IHC-P、IF-F |
M |
| Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (Rodent Specific) |
94885 |
WB、IP、IHC-P、 IF-IC、F |
M、R |
| PCNA (D3H8P) XP® Rabbit mAb |
13110 |
WB、IP、IHC-P、IF-F、 IF-IC、F |
H、M、R、Mk |
| Perforin (E3W4I) Rabbit mAb |
31647 |
WB、IHC-P |
M |
| α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb |
19245 |
WB、IP、IHC-P、IF-F |
H、M、R |
| Vinculin (E1E9V) XP® Rabbit mAb |
13901 |
WB、IHC-P、F |
H、M、R、Mk |
| 发育生物学 |
|||
| Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb |
19807 |
WB、IP、IHC-P、IF-F、 IF-IC、F |
H、M、R、Mk、 Z、Dg |
| HES1 (D6P2U) Rabbit mAb |
11988 |
WB、IP、IHC-P |
H、M、R、Mk |
| SMAD4 (D3R4N) XP® Rabbit mAb |
46535 |
WB、IP、HC-P、IF-IC、 F、ChIP、ChIP-seq |
H、M、R、Mk |
| Sox9 (D8G8H) Rabbit mAb |
82630 |
WB、IHC-P、IF-IC、F |
H、M、(M、R) |
| YAP (D8H1X) XP® Rabbit mAb |
14074 |
WB、IP、HC-P、IF-IC、 F、ChIP、ChIP-seq |
H、M、R、Hm、 Mk、(B、Hr) |
| 表观遗传学 |
|||
| ARID1A/BAF250A (D2A8U) Rabbit mAb |
12354 |
WB、IHC-P、ChIP |
H、M、R、Mk |
| EED (E4L6E) XP® Rabbit mAb |
85322 |
WB、IP、IHC-P、IF-IC、 F、ChIP |
H、M、R、Mk |
| Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb |
12041 |
WB、IP、HC-P、IF-IC、 F、ChIP、ChIP-seq |
H、M、R、Mk |
| 代谢 |
|||
| Phospho-AMPKα (Thr172) (D4D6D) Rabbit mAb |
50081 |
WB、IP、IHC-P |
H、M、R、C、Z、 B、Pg) |
| Arginase-1 (D4E3MTM ) XP® Rabbit mAb |
93668 |
WB、IHC-P、IF-F、 IF-IC、F |
H、M、R |
| COX IV (D6I4K) Rabbit mAb (Rodent Specific) |
38563 |
WB、IP、IHC-P |
M、R |
| SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific) |
23214 |
WB、IP、IHC-P、IF-IC |
M、R |
| 神经科学 |
|||
| Adora2a/Adenosine Receptor A2a (E5I8X) XP® Rabbit mAb (Rodent Specific) |
94871 |
IHC-P、IF-F |
M、R |
| GFAP (E4L7M) XP® Rabbit mAb |
80788 |
WB、IHC-P、IF-F |
H、M、R |
| Myelin Basic Protein (D8X4Q) XP® Rabbit mAb |
78896 |
WB、IHC-P、IF-F、IF-IC |
H、M、R |
| NCAM1 (CD56) (E7X9M) XP® Rabbit mAb |
99746 |
WB、IP、IHC-P、IF-F、 IF-IC、F |
H、M、R |
| NeuN (D4G4O) XP® Rabbit mAb |
24307 |
WB、IHC-P、IF-F |
H、M、R |
| Tau (D1M9X) XP® Rabbit mAb |
46687 |
WB、IHC-P、IF-F、IF-IC |
H、M、R |
| 免疫学 |
|||
| Btk (D3H5) Rabbit mAb |
8547 |
WB、IP、IHC-P、F |
H、M、(R、Hm、B、Dg、Pg、Hr) |
| CD3ε (D4V8L) Rabbit mAb |
99940 |
WB、IHC-P |
M |
| CD4 (D7D2Z) Rabbit mAb |
25229 |
WB、IHC-P |
M、R |
| CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific) |
98941 |
WB、IHC-P |
M |
| CD11c (D1V9Y) Rabbit mAb |
97585 |
WB、IHC-P、IF-F、IF-IC |
M |
| CD19 (D4V4B) XP® Rabbit mAb |
90176 |
WB、IP、IHC-P |
H、M、(B) |
| CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb |
77699 |
WB、IHC-P |
M |
| CD39/NTPDase 1 (E2X6B) XP® Rabbit mAb (Mouse Specific) |
14481 |
WB、IHC-P |
M |
| CD40 (E2Z7J) Rabbit mAb |
86165 |
WB、IP、IHC-P |
M |
| CD45 (D3F8Q) Rabbit mAb |
70257 |
P、IHC-P、IF-F、IF-IC |
M |
| F4/80 (D2S9R) XP® Rabbit mAb |
70076 |
WB、IP、IHC-P |
M |
| Granzyme B (E5V2L) Rabbit mAb (Mouse Specific) |
44153 |
WB、IP、IHC-P、F |
M |
| FoxP3 (D6O8R) Rabbit mAb |
12653 |
IHC-P、IF-F、F |
M |
| FcγRIIB (D8F9C) XP® Rabbit mAb(Mouse Specific) |
96397 |
WB、IP、IHC-P、F |
M |
| NT5E/CD73 (D7F9A) Rabbit mAb |
13160 |
WB、IHC-P |
H、M、R |
| PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) |
84651 |
WB、IP、IHC-P、IF-F、IF-IC、F |
M、(R、Hm) |
| PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) |
64988 |
IHC-P |
M |
| PD-L2 (D6L5A) Rabbit mAb |
49189 |
IHC-P |
M |
| S100A9 (D3U8M) Rabbit mAb (Rodent Specific) |
73425 |
WB、IP、IHC-P、 IF-F、F |
M、R |
| STING (D2P2F) Rabbit mAb |
13647 |
WB、IP、IHC-P |
H、M |
| TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific) |
83882 |
WB、IP、IHC-P |
M |
| VCAM-1 (D2T4N) Rabbit mAb (Mouse Specific) |
32653 |
WB、IHC-P |
M |